Teaching 2003/2004
Update: 19.05.2017

Links of interest
The course is managed online via WebCT.

Rasmol is a free molecular viewer available for PC, Mac and UNIX platforms.

Protein Explorer has been developed based on RasMol and is more powerful.
The software is free and runs on PC, Mac and Linux platforms.
Download of Chime may be required.

The PubMed Database of scientific literature: http://www.ncbi.nlm.nih.gov/PubMed/.
Contents
Tutorials
Biological Sciences 3rd year module: Structure and Functions of Proteins, 2003-2004
Tutorial T1, 10.10.03Amino acids, peptide bonds, spectroscopy
Downloads: Handout (PDF)
Tutorial T2, 31.10.03Protein Folding
Downloads: Handout (PDF)
Further reading, original literature and tutorials:
The hydrophobic effect from: Schutt & Lindberg (2000) Anat. Rec. 261, 198-216.
Tutorial T3, 28.11.03Enzyme Mechanisms
Downloads: Answers (PDF file)
Tutorial T4, 09.01.04Membrane Structure
Downloads: Handout (PDF file)
Tutorial T5, 23.01.04Bioenergetics
Downloads: Handout (PDF file)
Lectures
Biochemistry/Molecular Biology Honours: Structural Biology, 2003-2004
Structural Biology 8, 10.11.03The Structural Basis of Transcription: RNA Polymerase II
Downloads: Lecture (PDF), Lecture (6 slides per page; PDF), Handout (PDF)
Further reading, original literature and tutorials:
Cramer et al. (2000) Architecture of RNA polymerase II and implications for the transcription mechanism. Science 288, 640.
Cramer et al. (2001) Structural basis of transcription: RNA polymerase II at 2.8 Å resolution. Science 292, 1863.
Gnatt et al. (2001) Structural basis of transcription: An RNA polymerase II elongation complex at 3.3Å resolution. Science92, 1876.
Poglitsch et al. (1999) Electron crystal structure of an RNA polymerase II transcription elongationcomplex. Cell 98, 791.
Zhang & Darst (1998) Structure of the Escherichia coli RNA polymerase alpha subunit amino-terminal domain. Science 281, 262.
Zhang et al. (1999) Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 Å resolution. Cell 98, 811.
Structural Biology 9, 17.11.03The Structural Basis of Translation: The ribosome and protein synthesis
Downloads: Lecture (PDF), Lecture (6 slides per page; PDF), Handout (PDF)
Further reading, original literature and tutorials:
Beckmann et al. (2001) Architecture of the protein-conducting channel associated with the translating 80S ribosome. Cell 107, 361-372.
Gabashvili et al. (2000) Solution structure of the E. coli 70S ribosome at 11.5 Å resolution. Cell 100, 537-549.
Moore & Steitz (2002) The involvement of RNA in ribosome function. Nature 418, 229-235.
Wimberly et al. (2000) Structure of the 30S ribosomal subunit. Nature 407, 327-339.
Yusupov et al. (2001) Crystal structure of the ribosome at 5.5 Å resolution. Science 292, 883-896.
Biological Sciences 3rd year module: Structures and Functions of Proteins, 2003-2004
Lecture 7, 24.10.03Protein X-ray Crystallography (substituting for Malcolm Walkinshaw)
Downloads: Lecture (PDF), Handout (PDF)
Biotechnology Honours: Enzymology, 2003-2004
Enzymology 7, 05.11.03Membrane Enzymes
Downloads: Lecture (PDF), Handout (PDF)
Further reading, original literature and tutorials:
Web resource: Membrane Structure and Function (Cell Biology Webpages, Interactive learning topics in Cell Biology)
Web resource: Photosystem I (W Saenger, FU Berlin; some exciting pages about PSI structure and function)
Schubert et al. (1997) J. Mol. Biol. 272, 741-769.
Enzymology 8, 05.11.03Enzyme Encapsulation
Downloads: Lecture (PDF), Handout (PDF)
Further reading, original literature and tutorials:
Lasic (1998) Novel applications of liposomes. Trends Biotechnol. 16, 307.
Sawada & Ueda (2001) Enzyme processing of textiles in reverse micellar solution. J. Biotechnol. 89, 263.
Walde & Ichikawa (2001) Enzymes inside lipid vesicles: preparation, reactivity and applications. Biomol. Eng. 18, 143.
Electives
Structural Biology: Studying Macromolecules in Health and Disease, 2003-2004
Session 1, 06.01.04Lecture: Introduction, General Comments, UV/Vis Spectroscopy
Downloads: Handout (PDF file)
Session 2, 08.01.04Practical: Amino Acid Properties
Downloads: Handout (PDF file)
Session 3, 13.01.04Lecture: Fluorescence Spectroscopy 1
Downloads: Handout (PDF file)
Session 4, 15.01.04Lecture: Fluorescence Spectroscopy 2, Practical: Titration Experiments
Downloads: Handout (PDF file)
Session 5, 20.01.04Lecture: CD Spectroscopy, Practical: Analysis of CD spectra
Downloads: Handout (PDF file)
Session 6, 22.01.04Paper discussion
Downloads: Handout (PDF file)
Session 7, 27.01.04Practical: Fluorescence Experiment
Downloads: Results (PDF file), Literature 1 (PDF file), Literature 2 (PDF file)